منابع مشابه
Unique double-stranded fragments of bacteriophage T5 DNA resulting from preferential shear-induced breakage at nicks.
Nicks within one strand of the bacteriophage T5 DNA molecule act as " weak points" for a novel kind mechanical breakage that can be utilized for "dissecting" the genome. The products from sheared T5(+) DNA include five unique double-stranded segments of the molecule and various combinations of adjacent segments. These specific fragments are not obtained after repair of the nicks with DNA ligase...
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The " DNA-membrane complex" (1200S-400S) obtained by lysis of rat ascites hepatoma cells in a low concentration of sodium dodecyl sarcosinate-sodium deoxycholate sedimented through neutral sucrose gradient more rapidly than the free DNA" (200S) which was released by a high concentration of sodium dodecyl sulfate. The former was decomposed by Pronase in the gradient but the latter was not affect...
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The gene mutated in Nijmegen breakage syndrome, a chromosome instability disorder, has been identified and sequenced. The protein product of this gene forms a complex with hMre11 and hRad50--proteins that are involved in repairing double-strand breaks in DNA.
متن کاملShear unzipping of DNA.
We study theoretically the mechanical failure of a simple model of double-stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder model of shear unzipping proposed earlier by deGennes [de Gennes, P. G. C. R. Acad. Sci., Ser. IV: Phys., Astrophys. 2001, 1505]. Using this model and a combin...
متن کاملSpontaneous DNA breakage in single living cells of Escherichia coli
Generation of controls with a single DSB in a single chromosome per cell. When shifted to restrictive temperature, cells with a temperature-sensitive replication-initiation defective mutation, such as dnaA(TS), continue to divide until most contain a single chromosome [Supplementary Fig. 1]. We exploited this property to generate cells with a single chromosome so that we could assess the abilit...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1978
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(78)85396-x